|
KMID : 0381119800020010029
|
|
Genes and Genomics
1980 Volume.2 No. 1 p.29 ~ p.40
|
|
|
THE COMBINED EFFECTS OF UV-LIGHT AND METHYL METHANESULFONATE ON EXCISION REPAIR IN CULTURED HUMAN CELLS
|
|
PARK, SANG DAI/朴相大
CHOI, KYUNG HEE/HONG, SEUNG HWAN/崔慶喜/洪承煥
|
|
|
Abstract
|
|
|
DNA excision repair was measured in cultured normal human fibroblasts HF₁ after
dual treatments with UV-light and MMS in order to determine the repair pathways for
these agents. The results obtained were as follows: (1) The dose response of
unscheduled DNA synthesis induced by 10-30 J/㎡ UV-light or 0.5-5mM MMS in
normal human DNA synthesis in the combined treatment with MMS and UV-light was
found to be much less than the sum of those treated separately, and even less than that
produced by UV-light alone. This result indicates that MMS inhibits UV-induced
unscheduled DNA synthesis. (2) Pyrimidine dimers produced by 26 J/㎡ UV-light
continued to be excised until 48 hours, revealing 54% of the original dimers being
remained unexcised. However, in 1 mM or 2 mM MMS treated groups prior to
UV-irradiation, the unexcised dimer contents were found to be 69% and 80%,
respectively. These results indicate that MMS inhibits the excision of pyrimidine dimers
induced by UV-light.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|